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Primers for the second PCR include both a variable length sequence to increase library complexity and an 6 bp barcode for multiplexing of different biological samples: sense, 5-AAT GAT ACG GCG ACC ACC GAG ATC TAC ACT CTT TCC CTA CAC GAC GCT CTT CCG ATC T (19 bp variable length sequence) (6 bp barcode) tct tgt gga aag gac gaa aca ccg-3 and antisense, 5-CAA GCA GAA GAC GGC ATA CGA GAT AAG TAG AGG TGA CTG GAG TTC AGA CGT GTG CTC TTC CGA TCT tct act att ctt tcc cct gca ctg t-3

In this video, Im going to break down what size circle hook you should use for each type of bait, and some general guidelines to help you understand more about pairing circle hooks with live, cut or dead bait

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